Application of 3D-printed scabbard-like sorbent for sample preparation in bioanalysis expanded to 96-wellplate high-throughput format

Abstract

Modern bioanalysis, which involves the quantitative and qualitative determination of small-molecule endogenous and exogenous substances in biological samples, is a powerful and useful tool that can generate valuable information related to many areas connected with human health and quality of life. Although LC-MS and GC-MS are widely viewed as the gold standards for many bioanalytical tasks, the scientific community has not abandoned its search for newer, more efficient, and more inexpensive methods of performing extraction as a sample preparation step before final analysis. Recent research showing the immense potential of 3D printing compelled our group to explore how this technology could be applied to techniques used in analytical chemistry. In particular, 3D printing offers three promising advantages: availability, low cost of materials and equipment, and the ability to fabricate objects of nearly any shape to suit the needs of a given application. Previously, we demonstrated that a commercial 3D material (LAY-FOMM) can function as a chemically active object that enables the reversible sorption of the antidiabetic drug, glimepiride, and endogenous steroids. In this report, we use a 3D printer to fabricate sorbents with a scabbard-like shape for use with a 96-blade system, which, along with the use of a 96-well plate, allows multiple extractions to be performed simultaneously. In order to assess the relative benefits of this 3D printed approach, we compare the performance of the proposed LAY-FOMM-based sorbent to that of the widely used C18 sorbent. Although the LAY-FOMM sorbent showed lower extraction recovery rates than the C18 sorbent, all of the other validation parameters suggest that it is suitable for use in high-throughput analysis of steroids in human plasma.