Abstract
The article presents an overview of the main methods of obtaining biofilms in vitro, used in research. The technology of 3D-bioprinting is described – a new method in the modeling of bacterial biofilms, which solves one of the main problems in the study of biofilms- the heterogeneity of biomass, and opens up new opportunities for the study of various aspects of biofilm formation.
Highlights
There are two phenotypes of bacterial cultures: one is a single cell in a free-weighted state – the so – called planktonic form of bacterial culture, the second one is a complex of bacterial aggregates, called biofilm
Up to 99% of all bacteria exist in the form of biofilms – organized communities which represent a three-dimensional structure that dynamically changes in space and time, consisting of actively functioning and resting cells enclosed in an intercellular matrix synthesized by them and formed at the interface between phases [1]
Koch, which formed classical postulates [3], contributed to the study of pure cultures of bacteria that are phenotypically in planktonic form, and biofilms remained for a long time almost without any attention of researchers
Summary
There are two phenotypes of bacterial cultures: one is a single cell in a free-weighted state – the so – called planktonic form of bacterial culture, the second one is a complex of bacterial aggregates, called biofilm. Up to 99% of all bacteria exist in the form of biofilms – organized communities which represent a three-dimensional structure that dynamically changes in space and time, consisting of actively functioning and resting cells enclosed in an intercellular matrix synthesized by them and formed at the interface between phases [1]. Despite the fact that the dental plaque biofilm is probably the first preparation containing bacteria that was studied by microscopy and described by A. van Leeuwenhoek in 1684 [2], the further development of microbiology, and, in particular, the work of R. Koch, which formed classical postulates [3], contributed to the study of pure cultures of bacteria that are phenotypically in planktonic form, and biofilms remained for a long time almost without any attention of researchers
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