Abstract
A new strategy based on the use of the two-dimensional TOCSY experiment is described for the determination of the stoichiometry of association of strands in multi-stranded DNA structures. TOCSY spectra are recorded as a function of DNA concentration and the intensities of cross peaks are monitored to estimate the relative changes in the concentrations of the ordered and single-strand species at different total DNA concentrations. From these the stoichiometry and the association constant can be calculated. The method has been demonstrated on a quadruplex structure formed by the DNA segment d-AG3T. Copyright © 1999 John Wiley & Sons, Ltd.
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