Abstract
Main conclusionMethyl jasmonate promotes the synthesis of rosmarinic acid in Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib, and it promotes the latter more strongly.Salvia miltiorrhiza Bunge (SMB) is a traditional Chinese medicinal material, its water-soluble phenolic acid component rosmarinic acid has very important medicinal value. Salvia castanea f. tomentosa Stib (SCT) mainly distributed in Nyingchi, Tibet. Its pharmacological effects are similar to SMB, but its rosmarinic acid is significantly higher than the former. Methyl jasmonate (MJ) as an inducer can induce the synthesis of phenolic acids in SMB and SCT. However, the role of MJ on rosmarinic acid in SMB is controversial. Therefore, this study used SMB and SCT hair root as an experimental material and MJ as a variable. On one hand, exploring the controversial reports in SMB; on the other hand, comparing the differences in the mechanism of action of MJ on the phenolic acids in SMB and SCT. The content of related metabolites and the expression of key genes in the synthesis pathway of rosmarinic acid was analyzed by 1H-NMR combined with qRT-PCR technology. Our research has reached the following conclusions: first of all, MJ promotes the accumulation of rosmarinic acid and related phenolic acids in the metabolic pathways of SMB and SCT. After MJ treatment, the content of related components and gene expression are increased. Second, compared to SMB, SCT has a stronger response to MJ. It is speculated that the different responses of secondary metabolism-related genes to MJ may lead to different metabolic responses of salvianolic acid between the two.
Highlights
Salvia miltiorrhiza Bunge (SMB, Danshen in Chinese), belonging to Labiate family (Kai et al 2011; Shi et al 2014), is an important traditional Chinese herbal plant with long history for medicine as well as healthy food (Shi et al 2018)
Compared with SMB, the water-soluble component rosmarinic acid is higher in SCT, but the salvianolic acid B is lower (Yang et al 2009)
Hairy root of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib were obtained from an earlier experiment in our laboratory
Summary
Salvia miltiorrhiza Bunge (SMB, Danshen in Chinese), belonging to Labiate family (Kai et al 2011; Shi et al 2014), is an important traditional Chinese herbal plant with long history for medicine as well as healthy food (Shi et al 2018). There are two major types of pharmacologically active compounds present in SMB, one is water-soluble phenolic acids (Ma et al 2013), and the other is fat-soluble ketones (Kim et al 2008). Compared with SMB, the water-soluble component rosmarinic acid is higher in SCT, but the salvianolic acid B is lower (Yang et al 2009). SCT has important significance as a medicine source of rosmarinic acid
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