Abstract
The diagnosis of pathogenic bacteria in severe pneumonia is difficult and the prognosis is poor. Its outcome is closely related to bacterial pathogenicity and the timeliness and pertinence of antibiotic treatment. Therefore, early diagnosis is of great significance to the prognosis of patients. Sputum examination and culture is the gold standard for the diagnosis of pathogens of severe pneumonia. However, due to the long time of bacterial culture, the early use of antibiotics, the change of bacteria species, mixed infection and other problems, the results of bacterial culture in sputum are often false negative. With the continuous application of new molecular biology techniques in clinical detection, the classification of bacteria and microorganisms has deepened from the identification of phenotypic characteristics to the classification of gene characteristics. Sequencing analysis with 16S rDNA sequencing technology has the characteristics of high sequencing flux, large amount of data obtained, short cycle, and can more comprehensively reflect the species composition of microbial community, real species distribution and abundance information. In this paper, 16S rDNA sequencing technology was used to analyze the bacterial population composition in the sputum of severe pneumonia, and to explore a new method of etiological diagnosis.
Highlights
Due to the lack of typical symptoms and signs in the early diagnosis of experience after antibiotic treatment inappropriate often makes pneumonia in delay no more waiting for a reason, and gradually develops severe pneumonia, called toxic pneumonia or explosive pneumonia, which is the highest rates of pneumonia types [1]
Its outcome is closely related to bacterial pathogenicity and the timeliness and pertinence of antibiotic treatment, so early diagnosis is of great significance to the prognosis of patients [2]
The length of 16S rRNA is about 1500 bp, and it is often used as the basis for bacterial taxonomy studies. 16S rDNA sequencing analysis is characterized by high sequencing flux, large amount of data obtained, short cycle, and more comprehensive reflection of microbial community species composition, real species distribution and abundance information [3]. 16S rDNA sequencing technology is a bright spot in the development of modern molecular biology technology
Summary
16S rDNA sequencing analysis is characterized by high sequencing flux, large amount of data obtained, short cycle, and more comprehensive reflection of microbial community species composition, real species distribution and abundance information [3]. The sputum samples with positive 16S rDNA sequence were classified and identified by high-throughput sequencing technology, so as to find the common pathogenic bacteria of clinical infection of severe pneumonia and explore the new thinking of etiological diagnosis of severe pneumonia. Its sequencing analysis has the characteristics of high sequencing flux, large amount of data obtained, short cycle, and can more comprehensively reflect the species composition of microbial community, real species distribution and abundance information The purpose of this project is to use 16S RNA gene sequencing technology of bacterial ribosome small subunit (16S rDNA) to identify and classify bacteria in pneumonic sputum, and to explore a new method of etiological diagnosis. It is not possible to identify bacterial genus or species by V4, V5, V7 and V8 region sequences [8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
