Application Methodology and Physiological Insights of Melatonin Hormone for Water Stress Alleviation in Black Pepper (Piper nigrum L.)

Abstract

Black pepper is highly sensitive to water deficit stress especially during summer, resulting in significant losses in yield; therefore, strategies aimed at enhancing water stress tolerance are essential. Melatonin improves stress tolerance in plants; however, its method of application and optimum concentration in black pepper under water deficit stress remains unclear. Therefore, we conducted a two pot culture experiment during March and April, 2022 (var. Panniyur-1) to investigate the effects of foliar-sprayed and root-irrigated melatonin (50, 100 and 150 µM L-1) on the recovery per cent and physiological mechanism under water stress. The treatment details were, WW - Well-watered; WS - Water stressed; FM50 - Waterstress+50µM Melatonin (Foliar spray); FRM50 - Water stress + 50µM Melatonin (Foliar spray + Soil drenching @ 50ml/plant); FM100 - Water stress + 100µM Melatonin (Foliar spray); FRM100 - Water stress + 100µM Melatonin (Foliar spray + Soil drenching @ 50ml/plant); FM150 - Water stress + 150µM Melatonin (Foliar spray); FRM150 - Water stress + 150µM Melatonin (Foliar spray + Soil drenching @ 50ml/plant). The melatonin-induced enhanced stress tolerance could be attributed to improved recovery %, leaf relative water content, photosynthetic pigments, activity of antioxidant enzymes (SOD, POD, and CAT), and ultimately significantly relieved the inhibitory effects of water stress on leaves. After rehydration, melatonin-treated plants recovered more quickly than untreated plants. In addition, melatonin counteracted the water stress induced accumulation in proline content. Overall, the results of this study demonstrated that melatonin at 100 µM L-1 (Foliar spray and root irrigation) significantly alleviated the adverse effects of water deficit stress compared untreated plants. In addition, application of exogenous melatonin combined with root and foliar application is superior than foliar spraying alone.