Abstract

Root-knot nematode is a kind of omnivorous plant pathogenic nematode that causes great harm to underground roots and above-ground parts of plants, and has become a serious factor restricting the development of global agriculture. In order to realize the sustainable development of agriculture, the research carried out an in-depth discussion on biological control methods. Firstly, a bacillus was obtained through insecticidal experiments, then a large amount of materials was obtained through purification and cultivation, and then the stability test of the active substance of Bacillus was conducted to explore its influence to root-knot nematodes. Finally, the pot verification experiment and field verification experiment were conducted. The results showed that in the insecticidal experiment, the accumulation of active substances at 14 h was the maximum, and the corresponding mortality of root-knot nematodes at 12 h and 24 h was 97.6% and 82.91%, respectively. In the BS pot experiment, the number of tomato root knot formation in the bacillus treated group was significantly reduced by 46.3% compared with the control group, and the number of egg sac of the rhizome was reduced by 78.31%. On the 120th day of field verification experiment, the fruit quantity and yield per plant in the treatment group increased by 17.02% and 11.85%, respectively. In summary, Bacillus can effectively reduce the damage of root knot nematode to tomato plants, and has a good prospect of application and popularization.

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