Abstract
Limonium brasiliense is a common plant on the southern coast of Brazil. The roots are traditionally used for treatment of premenstrual syndrome, menstrual disturbances and genito-urinary infections. Pharmaceutical preparations obtained from its roots and used for these purposes were marketed in Brazil in the 1980s and 1990s. Currently, the Brazilian Drug Agency (National Health Surveillance Agency, ANVISA) has canceled the registration of these products, and their use was discontinued because of a lack of studies to characterize the plant raw material and ensure the effectiveness and safety of its use. The aim of the present study was to develop and validate an analytical method to determine the content of total polyphenols (TP) in an extract from L. brasiliense roots, by the UV/Vis spectrophotometric method. L. brasiliense roots were extracted in acetone:water (7:3, v/v-10% w/v). The crude extract was used to develop a method for TP assay. The method was validated according to national and international guidelines. The optimum conditions for analysis time, wavelength, and standard substance were 30 min, 760 nm, and pyrogallol, respectively. Under these conditions, validation by UV/Vis spectrophotometry proved the method to be linear, specific, precise, accurate, reproducible, robust, and easy to perform. This methodology complies with the requirements for analytical application and to ensure the reliability of the results.
Highlights
Phenolics include simple phenols, phenolic acids, coumarins, flavonoids, stilbenes, hydrolyzable and condensed tannins, lignans, and lignins
Among the many varieties of natural phenolic compounds, the procyanidins are an important subgroup. These substances are composed of oligomers and polymers that consist of catechin and/or epicatechin units linked mainly through C4→C8 and/or
The statistical analysis indicated a significant increase in absorbance at 760 nm compared to data for 691 and 715 nm for all solutions
Summary
Phenolic acids (benzoic and cinnamic acid derivatives), coumarins, flavonoids, stilbenes, hydrolyzable and condensed tannins, lignans, and lignins. These compounds are among the most widely occurring secondary metabolites in the plant kingdom, acting mainly as phytoalexins, attractants for pollinators, contributors to plant pigmentation, antioxidants, and protective agents against UV light, among others [1]. Among the many varieties of natural phenolic compounds, the procyanidins are an important subgroup. These substances are composed of oligomers and polymers that consist of catechin and/or epicatechin units linked mainly through C4→C8 and/or. Assuming that quantification of individual polyphenols does not adequately reveal the proportion of polymeric procyanidins, spectrophotometry in the ultraviolet region may be a useful tool to help resolve this problem [8,9]
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