Abstract
Summary The ribotype analysis of a collection of twenty nine Photobacterium damsela subsp. piscicida strains, with different host and geographical origins, was performed in order to analyze the possible genetic variability within this bacterial fish pathogen. In addition, the value of this technique for intraspecific classification and epidemiological studies of Ph. damsela subsp. piscicida was evaluated. After restriction with four endonucleases, Pvu I Pvu II, Sma I, and Hind III, two major ribotypes could be established. The first ribopattern compiled the European strains, while the second ribotype included the Japanese isolates. A third ribotype was observed with the enzymes Pvu I, Sma I and Hind III, constituted in all cases by an unique strain. Endonuclease Pvu I clearly differentiated the french isolate, while Sma I and Hind III distinguished the Israeli strain from the other European Ph. damsela subsp. piscicida . The similarities among ribotypes, calculated by the Dice coefficient, varied from 30–40%, with Pvu I and Pvu II, to 80–90% with Sma I and Hind III. The results obtained indicate the usefulness of the ribotyping procedures for epidemiological studies and also as an important taxonomic tool for the fish pathogen Ph. damsela subsp. piscicida .
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