Abstract
Among the fungal diseases of apple trees, serious yield losses are due to an apple scab caused by Venturia inaequalis. Protection against this disease is based mainly on chemical treatments, which are currently very limited. Therefore, it is extremely important to introduce cultivars with reduced susceptibility to this pathogen. One of the important sources of variability for breeding is the process of polyploidization. Newly obtained polyploids may acquire new features, including increased resistance to diseases. In our earlier studies, numerous tetraploids have been obtained for several apple cultivars with ‘Free Redstar’ tetraploids manifesting enhanced resistance to apple scab. In the present study, tetraploids of ‘Free Redstar’ were assessed in terms of phenotype and genotype with particular emphasis on the genetic background of their increased resistance to apple scab. Compared to diploid plants, tetraploids (own-rooted plants) were characterized with poor growth, especially during first growing season. They had considerably shorter shoots, fewer branches, smaller stem diameter, and reshaped leaves. In contrast to own-rooted plants, in M9-grafted three-year old trees, no significant differences between diplo- and tetraploids were observed, either in morphological or physiological parameters, with the exceptions of the increased leaf thickness and chlorophyll content recorded in tetraploids. Significant differences between sibling tetraploid clones were recorded, particularly in leaf shape and some physiological parameters. The amplified fragment length polymorphism (AFLP) analysis confirmed genetic polymorphism of tetraploid clones. Methylation-sensitive amplification polymorphism (MSAP) analysis showed that the level of DNA methylation was twice as high in young tetraploid plants as in a diploid donor tree, which may explain the weaker vigour of neotetraploids in the early period of their growth in the juvenile phase. Molecular analysis showed that ‘Free Redstar’ cultivar and their tetraploids bear six Rvi genes (Rvi5, Rvi6, Rvi8, Rvi11, Rvi14 and Rvi17). Transcriptome analysis confirmed enhanced resistance to apple scab of ‘Free Redstar’ tetraploids since the expression levels of genes related to resistance were strongly enhanced in tetraploids compared to their diploid counterparts.
Highlights
The most important fruit species in the temperate climate zone is the apple tree (Malus × domestica Borkh.)
Rvi5, Rvi11, and Rvi14 are considered to confer durable apple scab resistance and Rvi6 has been estimated to be useful in resistance breeding as well, since its resistance is overcome by the pathogen with medium frequency [4]
The activities of various genes related to resistance were strongly enhanced in tetraploids of the ‘Free Redstar’ apple cultivar, as evidenced by their significantly increased expression levels compared to their diploid counterparts
Summary
The most important fruit species in the temperate climate zone is the apple tree (Malus × domestica Borkh.). Among the fungal diseases of apple trees, serious yield losses are caused by apple scab, of which the causal agent is the ascomycete fungus Venturia inaequalis [1]. Protection against this disease is based mainly on chemical treatments and accounts for most of the total cost of protection against pests and diseases [2]. It is extremely important to introduce cultivars with increased resistance to this disease into production [1,4]
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