Abstract

Disinfected seeds of soybean and actively growing cultures of Chlorella vulgaris were grown on nitrogen-free media. The nitrate content of both the soybean seedlings and algal cultures substantially increased during nitrogen starvation. The nitrate level in soybean seedlings was at least eight times greater than seeds after two to three weeks. Nitrogen starvation also caused an increase in the nitrate content of the algal cultures. Nitrate reductase activity also increased, and its appearance was sensitive to cycloheximide. Tungstate, added during starvation, inhibited the induction of nitrate reductase with a concomitant increase in the level of nitrate. These data suggest that oxidation of reduced nitrogen compounds can occur in higher plants and algae.

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