Abstract
The effect of immune interferon (IFN-γ) and recombinant tumor necrosis factor (rTNF-α) on cellular differentiation was investigated in human promyelocytic leukemia cell line HL-60. Both IFN-γ and rTNF-α induced the appearance of the monocytic phenotype in a dose- and time-dependent manner as assessed by morphology, reduction of nitroblue tetrazolium and the induction of α-naphthyl butyrate esterase. Utilizing a nondenaturing polyacrylamide electrophoretic assay, it was revealed that a membrane-bound tyrosine kinase activity accompanied the appearance of the differentiated cell type. These results suggest that the induction of membrane-bound tyrosine kinase activity by IFN-γ and rTNF-α may be an important characteristic of monocytic differentiation.
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