Appearance of a class of cell-surface fucosyl-glycopeptides in differentiated muscle cells in culture

Abstract

Fucosyl-glycopeptides synthesized in culture by duplicating myoblasts and multinucleated myotubes were partially resolved by gel-filtration on Sephadex G-50 in two main components with K av of 0.3 and 0.6, respectively. DEAE-cellulose chromatography of fucosyl-glycopeptides resolved several components common both to myoblasts and myotubes; however an acidic component, eluted at 24 m M Na-phosphate, is present only in multinucleated myotubes. Neuraminidase treatment of this component abolished its affinity for DEAE-cellulose indicating that its anionic properties are due to the presence of sialic acid residues. Its location on the outer myotube plasma membrane is suggested by the observation that this acidic glycoconjugate was also found in the glycopeptide fraction released by mild trypsin treatment of intact cells in culture. This component appears heterogeneous since it was resolved on Sephadex G-50 into two main peaks corresponding to those obtained by gel-filtration of total glycopeptides. Differentiated postmitotic myoblasts, whose fusion has been inhibited by low Ca 2+ concentration, synthesize the specific anionic glycopeptides whereas BrdU-treated myoblasts do not. Culture conditions have no effect on the synthesis of these glycopeptides, since myoblasts grown in conditioned medium, collected from myotube cultures, or myoblasts, grown at high cell density, do not synthesize this class of acidic glycopeptides.