Abstract
Coxiella burnetii is a highly infectious zoonotic pathogen infecting wide range of mammals, including humans. In the present study, a total of 711 blood samples from bovines [cattle (n = 543) and buffaloes (n = 168)] from eight farms at different geographical locations in India were screened for C. burnetii targeting the IS1111 and the com1 genes. The anti-C. burnetii antibodies in serum samples were detected using indirect-ELISA kits. Also, a total of 21 parameters pertaining to animal health and farm management were identified to assess their role as possible risk factors for coxiellosis among the targeted farms. The apparent prevalence (positive for PCR and/or ELISA) for coxiellosis was reported to be 24.5% in cattle and 8.9% in buffaloes. In cattle, the detection rate of C. burnetii employing the IS1111 gene (8.5%) was found to be significantly higher (p<0.05) as compared to the com1 (6.5%) gene. The seropositivity by ELISA was higher among cattle (17.7%) than in buffaloes (8.3%). Further, on univariable analysis of risk factors, species (cattle) (OR:3.31; 95%CI:1.88–5.82), inadequate floor spacing (OR:1.64; 95%CI:1.10–2.43), mastitis (OR:2.35, 95%CI:1.45–3.81) and reproductive disorders (OR:2.54; 95%CI:1.67–3.85) were significantly (p<0.05) having high odds for coxiellosis. The multivariable logistic regression analysis of the animal level risk factors revealed that species and age were found to be significantly associated with coxiellosis. However, since the number of screened farms is limited; further research is needed with a higher number of animals to confirm the farm level odds ratio of risk factors. Quarantine and biosecurity measures including farm hygiene operations were observed to be inadequate and also the lack of awareness about coxiellosis among the farm workers. In absence of vaccination program for coxiellosis in India, robust surveillance, farm biosecurity measures and the awareness for the disease among risk groups can play an important role in the disease prevention and subsequent transmission of the pathogen.
Highlights
Coxiellosis, a zoonosis of public health concern, is caused by Gramnegative intracellular bacterium Coxiella burnetii [1]
Information on the epidemiology of C. burnetii in animals is inadequate in India
The study appears to be first of its kind in India which involves bovines from different states to assess the apparent prevalence of coxiellosis at farms including the associated risk factors
Summary
Coxiellosis ( known as Q fever), a zoonosis of public health concern, is caused by Gramnegative intracellular bacterium Coxiella burnetii [1]. The disease causes significant impact on public health as well as socio-economic structure of the animal husbandry sector. The prevalence in these countries has been reported around 25% and the infected animals are the major sources of infection to farmers and other contact groups [4]. The Netherlands outbreak (2007–2010) of Q fever provided a clear demonstration of the serious threat posed to the public health in the absence of adequate diagnostic, therapeutic and epidemiological tools [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
