Abstract
Cell-free culture supernatant fractions derived from cultures ofHaemophilus parasuis andH. pleuropneumoniae failed to catabolize NAD irrespective of the presence or absence of dithiothreitol and/or arginine methyl ester. Although NAD was catabolized by resting cell suspensions of either organism and NMN, nicotinamide riboside, nicotinamide, adenosine, and adenine were detected as reaction products, the presence of dithiothreitol and/or arginine methyl ester in the reaction mixture made no obvious difference, qualitatively or quantitatively, to the results obtained. It is concluded that the production of nicotinamide from NAD by these organisms is due to the activities of conventional (i.e., nontoxigenic) enzymes rather than to toxins exhibiting ADP-ribosyltransferase activity.
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