Abstract
Mithramycin is shown to be able to reveal details of heterochromatin organization in interphase and dividing nuclei of higher plants. It has been used to show that in hyphal tip nuclei of Saprolegnia ferax the chromatin does not undergo any detectable condensation until telophase when it appears to contract into two homogeneous polar masses. In contrast, presumptive meiotic prophase nuclei in antheridia did contain condensed chromatin. These results suggest that the chromatin of S. ferax does not undergo a normal cycle of condensation during the prophase and metaphase stages of the mitotic cycle. Mithramycin also has the potential to help preselect desired nuclei prior to electron microscope studies.
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