Abstract
An apparatus which rapidly provides and maintains constant oxygen partial pressure from 1 to 150 Torr (1.4 to 211 μ m) in suspensions of respiring rat hepatocytes (0.50 – 0.87 μ m s −1) was developed. Voltage output from a Clark-type electrode in an incubation vessel regulates oxygen flow into the vessel to control solution oxygen concentration at a predetermined level. After maintenance of cell suspensions (4–15 ml) for 10 to 30 min, metabolism can be quenched and examined.
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