Apoptotic with Double-Staining Test, P53, and Cyclooxygenase-2 to Proliferation Colon Cancer Cell (WiDr) of Dolichol in Three Mangrove Leaves

Abstract

BACKGROUND: Mangroves secondary metabolites are mostly consisting of sterols, ubiquinones, isoprenoids, and polyisoprenoids. Polyisoprenoid is divided into two types, namely, polyprenol and dolichol, which has been reported to have biological and pharmacological activities.
 AIM: This research was aimed to analyze apoptosis 48 h with double staining and immunocytochemistry (ICC) 48 h of P53 and cyclooxygenase-2 (COX-2) gene expression from chemical constituents of dolichol in three mangrove leaves of Ceriops tagal, Nypa fruticans, and Rhizophora mucronata.
 METHODS: Apoptosis with the double-staining method was employed to analyze the genes expression in growth and development of cancer cells, P53, and COX-2 with ICC and flow cytometry method. The data were statistically analyzed using one-way ANOVA parametric statistical analysis followed by Duncan’s test.
 RESULTS: The result revealed that the increased apoptosis of samples C. tagal was 70% fluorescence orange, while N. fruticans and R. mucronata were 35% and 30% fluorescence orange, respectively. However, it was compared with the positive control; it produced orange fluorescent as much as 75%, suggesting that C. tagal have a position similar to 5-FU. Predominance dolichol in N. fruticans and C. tagal leaves led the expression gene of p53 to have 1.57% M1 phase, indicating the domination in G0-G1 phase (70–80%). Inhibit the expression for 48 h in p53 and COX-2 showing that n-hexane extract of C. tagal had the most percentage (80.733 ± 0.11%) to upregulate the p53 and less percentage (20.16 ± 1.19%) to downregulate the COX-2, indicating positive extract belong to N. fruticans and R. mucronata leaves.
 CONCLUSION: The present study confirmed the pharmacological properties of dolichol from three mangrove leaves as an anticancer of tumor suppressor genes and significantly proliferated of cancer cell growth from mangrove leaves.