Apoptotic response to acute and chronic exercises in rat skeletal muscle: Eccentric & sprint interval

Abstract

AimsApoptosis is a type of cell death that is vital for tissue homeostasis. Exercise can lead to initial stimulation of apoptotic regulator genes. We investigated their response to an acute exercise and their adaptations to chronic exercise training with an emphasis on eccentric and sprint interval exercises. Main methodsMale Sprague Dawley rats were randomly assigned to five groups (n = 8): acute eccentric exercise (AEE), acute sprint interval exercise (ASE), chronic eccentric exercise (CEE), chronic sprint interval exercise (CSE) and control (C). The AEE group underwent downhill running (at −16° slope) at 16 m/min 18 sets. The ASE group run for 7 sets and the speed increased gradually to 70–80 m/min. The chronic groups were implemented for 9 weeks. The CEE run 1 set for 15 min at −4° slope that increased gradually to 90 min at −16°. The CSE sprinted 1 min with 2–5 min rest. The mRNA in soleus (slow-twitch muscle) and super vastus lateralis (SVL) (fast-twitch muscle) muscles was analyzed by real-time RT-PCR. Key findingsAccording to the gene expression level in soleus muscle, apoptotic responses to acute and chronic sprint interval exercise as well as towards chronic eccentric exercise were clearly evident. But in SVL muscle, the only acute eccentric exercise group showed significance increase in apoptotic factors. Significancethese results revealed the apoptotic response to the exercise depends on the type and intensity of exercise and also on the sensitivity and susceptibility of the muscle.