Abstract
STAMP genes STAMP1/STEAP2 and STAMP2/STEAP4 are expressed in androgen receptor positive-prostate cancer cell line LNCaP, and this regulation was reported, previously. The androgen induction was done at LNCaP cells. STAMP2/STEAP4 was silenced for 1, 2 and 5 days. Using real-time RT-PCR, an apoptosis panel including proapoptotic and/or apoptotic genes was investigated for gene expression alterations in STAMP expressing LNCaP cells in comparison to P53-null PC3 cells in which STAMP2 gene was transfected. In addition, promoter region of the STAMP2 gene was analyzed and putative p53 and androgen receptor (AR) response elements were identified. Thus, p53 gene was silenced using siRNA approach and was induced by hydrogen peroxide. Taken together, prostate-specific STAMP2 gene and its regulation with the p53- and caspase-related pathway was characterized. These results will shed light on understanding the control of proliferation in neoplastic transformation in prostate cancer.
Highlights
The effect of androgens are mediated by androgen receptor (AR) in prostate, most of the studies focus on identification of AR regulated genes that might be highly expressed in the prostate tissue and its cancer
STAMP genes STAMP1/STEAP2 [1] and STAMP2/STEAP4 are expressed in androgen receptor positive-prostate cancer cell line LNCaP and their androgen receptor-mediated regulation was reported previously [2]
PC3 cells transfected with STAMP constructs (STAMP1, 2 and 3 versus HisMax-vector)
Summary
The effect of androgens are mediated by AR in prostate, most of the studies focus on identification of AR regulated (target) genes that might be highly expressed in the prostate tissue and its cancer. STAMP genes STAMP1/STEAP2 [1] and STAMP2/STEAP4 are expressed in androgen receptor positive-prostate cancer cell line LNCaP and their androgen receptor-mediated regulation was reported previously [2]. Their role in metabolic disease and essential position of STAMP2 for prevention of excessive inflammation were reported in mice studies [3]. STAMP2/ TIARP expression induction with interleukin-1-beta was demonstrated for the first time especially in human adipocyte cell model [4] Another member of the STAMP family includes pHyde, a rat protein that has been implicated in apoptosis of prostate cancer cells [5]. Studies reported that STAMP family members have metalloreductase activities associated with iron and copper uptake into HEK293T cells [7], though mentioned activities have not been shown for prostate cells, yet
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