Abstract
BackgroundApoptosis is a process of programmed cell death, and apoptosis defect results in serious diseases such as cancer. Apoptosis induction is one of the key mechanisms of anti-cancer agents. This study was aimed to find anti-prostate cancer compounds from marine-derived fungus Microsporum sp.ResultsWe found that physcion isolated from the fermentation broth extract of the marine fungus Microsporum sp. strain MFS-YL decreases the cell proliferation of PC3 human prostate cancer cells. Physcion induced cell apoptosis as determined by Annexin V/propidium iodide double staining. Physcion downregulated the anti-apopotoic proteins such as Ras, Bcl-xL, and Bcl-2, whereas upregulated the pro-apoptotic Bax. Physcion also activated caspase-3, caspase-8, and caspase-9.ConclusionThese results suggest that physcion from Microsporum sp. inhibits the proliferation of PC3 human prostate cancer cells via the pathway leading to apoptotic cell death. Physcion may be a potential candidate in the field of anticancer drug discovery against human prostate cancer.
Highlights
Apoptosis is a process of programmed cell death, and apoptosis defect results in serious diseases such as cancer
As part of an effort to discover novel anti-prostate cancer compounds from marine organisms, we have investigated the bioactive constituents of the marine-derived fungus Microsporum sp. strain MFS-YL
Physcion isolated from marine fungus Microsporum sp. inhibits the proliferation of human prostate cancer PC3 cells To identify compounds with cytotoxic effect against human advanced prostate cancer cells from marine-derived Microsporum sp., some compounds (2.48 g) were purified from a pool of secondary metabolites
Summary
Apoptosis is a process of programmed cell death, and apoptosis defect results in serious diseases such as cancer. Apoptosis induction is one of the key mechanisms of anti-cancer agents. This study was aimed to find anti-prostate cancer compounds from marine-derived fungus Microsporum sp. The proliferation inhibition and apoptosis induction of cancer cells have been used as a marker for evaluation of phytochemical anticancer activities Lowe and Lin 2000; Gurumuthy et al 2001). As part of an effort to discover novel anti-prostate cancer compounds from marine organisms, we have investigated the bioactive constituents of the marine-derived fungus Microsporum sp. Most dangerous advanced prostate cancer cells are resistant to apoptotic cell death and do not well respond to cytotoxic chemotherapeutic agents (Feldman and Feldman 2001; Grossmann et al 2001). We isolated physcion demonstrating anti-proliferation activity against PC3 human advanced prostate cancer cells. Physcion is an anthraquinone derivative and has been reported to inhibit proliferation of colorectal cancer cells HCT116 and
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
