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Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most serious cancers worldwide. Even with recent developments, there has been slight progress in improving the survival of HCC patients. Current therapies are accompanied by huge side effects beside incomplete recovery. Therapy by Herbal shows a marked beneficial effect. Aim: Our study screening of the cytotoxic effect of R. stricta nanoparticles which locally known as “Harmal” against highly aggressive cancer cells (in vitro). Procedures: R. Stricta Nano particles was gained by undergoing R. Stricta sheets to ball milling process to convert particles into Nano scale diameter. Cytotoxicity of different concentrations of nano- Harmal (0.0, 100 and 500 µg/ml) effects on the mRNA expression of Bax and Bcl-2 apoptosis related genes in Hep G-2 and Huh-7cell lines were investigated using real-time quantitative PCR analysis. and MTT assay and FACS analysis Results: It is clear from SEM images that the Harmal nanoparticles are predominantly spherical in shape and polydispersity with particle size around 100 nm. Screening of of Harmal nanoparticles (HNP) after 24 and 48 hours showed a significant cytotoxic effect on all cell lines at 500 μg/ml (p < 0.05) but has a high significant decrease in Hep G-2 cell viability with 100 and 500 μg/ml of HNP Hr(p < 0.001) of treatment compared with other cell lines. Conclusion and clinical relevance: Moving on gene expression and MTT assay and FACS analysis, we concluded that the HNP produced a clear cell death apoptosis in Hep G-2 and Huh-7 cell lines
Highlights
Hepatocellular carcinoma (HCC)was considered the ninth cause of death around the world [1]
Current therapies are accompanied by marked side effects beside incomplete recovery
Stricta sheets to ball milling process to convert particles into Nano scale diameter which examined by scanning electron microscopy (SEM)
Summary
Hepatocellular carcinoma (HCC)was considered the ninth cause of death around the world [1]. Both prolonged liver disease and cirrhosis are the major factors for HCC initiation and progression. There is no effective treatment at this stage and the morbidity rate become high and irrespective of the recovery therapy, HCC patients require a multidisciplinary therapy to achieve the optimal outcome [5]. Cytotoxicity of different concentrations of R.stricta nanoparticles (0.0, 100 and 500 μg/ml) was evaluated via its effects on the mRNA expression of Bax and Bcl-2 apoptosis related genes in Hep G-2 and Huh7cell lines using real-time quantitative PCR analysis, MTT assay and FACS analysis Results: It is clear from SEM images that R.Stricta nanoparticles are predominantly spherical in shape and polydispersity with particle size around 100 nm. Conclusion and clinical relevance: Moving on gene expression,MTT assay and FACS analysis, it could concluded that the R. stricta, nanoparticles produced apoptosis in Hep G-2 and Huh-7 cell lines
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