Abstract
Objective To investigate the feasibility of apoptosis detection in vivo by 99mTc-hydrazino nicotinamide(HYNIC)-Annexin V.Methods Annexin V was labeled with 99mTc through HYNIC.Ten New Zealand rabbits implanted with VX-2 were divided into control (C,n =4) and paclitaxel (PAC,n =6) groups,given 2 mL/kg of normal saline or 2.4 mg/kg of PAC intravenously.Images of the liver tumor were examined by single photon emission computed tomography (SPECT) through intravenous injection of99mTc-HYNIC-Annexin V before and 24 and 48 h after chemotherapy.Tumor radioactive count proportion to non-tumor sites (T/NT) was calculated.When last imaging finished,the rabbits were sacrificed.The tumor was taken out and divided into two pieces for TdT-mediated dUTP nick end labeling (TUNEL) immunohistochemical analysis and flow cytometry (FCM) respectively.Data were analyzed by t test,variance analysis and correlation analysis by SPSS 17.0.Results The labeling rate of Annexin V with 99mTc through HYNIC was above 95％,and radiochemical purity was above 95％.The SPECT showed that T/NT in control group and PAC group was 2.62 ± 0.56 and 2.53 ± 0.60 before chemotherapy,and that in control group and PAC group at 24 and 48 h after chemotherapy was 2.72 ±0.70 and 2.62 ±0.66,and 6.43 ±0.97 and 6.64 ± 1.15 respectively.The T/NT in PAC group at 24 and 48 h after chemotherapy was significantly different from that in control group and PAC group prior to chemotherapy (P ＜ 0.01).There was no significant difference between 24 h and 48 h in PAC group (P ＞ 0.05).The tunnel positive cells detected by immunohistochemistry and apoptosis rate detected by FCM in PAC group [(13.50 ± 2.74) ％ and (20.81 ± 3.33) ％ were significantly different from those in control group (4.00 ± 0.81)％ and (7.83 ± 1.11)％,P ＜ 0.01].The T/NT was significantly correlated with TUNEL positive cells and apoptosis rate of the tumor (rTUNEL =0.747,P ＜ 0.05 ; rFCM =0.909,P ＜ 0.01).Conclusion PAC can induce apoptosis of VX-2 cells in the liver of rabbits.Apoptotic cells in vivo can be detected by SPECT through 99mTc-HYNIC-Annexin V.24-48 h after chemotherapy is a window time for detecting apoptosis. Key words: Carcinoma, hepatocellular; 99mTc-hydrazino nicotinamide-Annexin V ; Apoptosis ; VX-2
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