Abstract
Objective To verify whether anandamide (AEA) has a capacity of inducing apoptosis in a HCC cell line with highly metastatic potential (MHCC97H), and investigate the role of Caspase-3 in apoptosis. Methods MHCC97H cells were treated with AEA at the different concentrations (0,50 or 100 μmol/L, respectively), and apoptosis was tested by Annexin V-FITC Apoptosis Detection Kit. Caspase-3 ac-tivity was measured by Caspase-3 Fluorescent Assay Kit, and the Caspase-3 mRNA, bcl-2 and bax were de-tected by reverse transcription polymerase chain reaction (RT-PCR). Results After treatment with AEA (100 μmol/L) for 24 h, apoptosis rate was (81.35±9.71)%, and Caspase-3 activity in MHCC97-H cells was increased significantly from 142.0±5.8 to 226.0±6.4 (P<0.01). There was significant difference in the expression of Caspase-3 mRNA between AEA group (0.54±0.32) and control group (0.24±0.13) following 24 h of treatment (P<0.01). The mRNA expression of bcl-2 and bax could not be detected in MHCC97-H cells between AEA group and control group. Conclusion The apoptosis of MHCC97H cells could be induced by AEA via Caspase-3-transducted signal. Key words: Carcinoma,hepatocellular; Apoptosis; Anandamide; Caspase-3
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
