Apoptosis inducing effects of Kuding tea polyphenols in human buccal squamous cell carcinoma cell line BcaCD885.

Xin Zhao,Liang Pang,Li-Hua Qiu,Jia-Le Song,Jing Li

doi:10.3390/nu6083084

Abstract

Tea polyphenols are functional substances present in tea. Kuding tea as a traditional drink also contains these compounds. After 25, 50 and 100 μg/mL of Kuding tea polyphenol treatment for 48 h, cell proliferation of human buccal squamous cell carcinoma cell line BcaCD885 was inhibited, and the 100 μg/mL of Kuding tea polyphenol showed the highest inhibitory rate at 72.3%. Compared to the lower concentration, the 100 μg/mL of Kuding tea polyphenols significantly (p < 0.05) induced apoptosis as determined by flow cytometry analysis, the content of sub-G1 cancer cells was 32.7%. By RT-PCR and western blot assays, Kuding tea polyphenol significantly induced apoptosis in BcaCD885 cancer cells (p < 0.05) by upregulating caspase-3, caspase-8, caspase-9, Fas/FasL, Bax, p53, p21, E2F1, p73 and downregulating Bcl-2, Bcl-xL, HIAP-1, and HIAP-2 mRNA and protein expressions. Kuding tea polyphenols thus present apoptosis inducing effects in vitro.

Highlights

Kuding tea, as a type of traditional pure natural health beverage, is prepared from leaves of “Kudingcha”, a Holly, which is a type of Aquifoliaceae evergreen tree
The different concentration of Kuding tea polyphenol showed similar cell growth numbers as the control, but after 2 days of incubation, growth of treated cells was gradually inhibited in a concentration-dependent manner
Kuding tea polyphenol treated cancer cells were markedly inhibited compared to control cells; 25 and 50 μg/mL Kuding tea polyphenol had inhibitory effect, but weaker than the 100 μg/mL

Summary

Introduction

As a type of traditional pure natural health beverage, is prepared from leaves of “Kudingcha”, a Holly, which is a type of Aquifoliaceae evergreen tree. It is mainly produced in the southwestern and southern region of China [1]. According to traditional Chinese medicine, Kuding tea has the effects of lowering blood pressure, maintaining proper weight, and removing blood stasis. It has the function of anti-cancer and anti-aging [3,4].

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Discussion

Conclusion