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Abstract
In this communication our aim was to study the JK cell growth inhibitory and apoptosis-inducing effects of ginsenoside Rg6 (GRg6) from steamed notoginseng on human lymphocytoma. The CCK-8 method was used to observe the anti-proliferative effect of GRg6 on human lymphocytoma JK cells. Flow cytometry was performed to analyze the influence of GRg6 on cell cycle. The Annexin-V FITC/PI double-staining method was used to detect the ratio of apoptotic cells. JC-1 staining was undertaken to observe the influence of GRg6 on intracellular mitochondrial membrane potential. Finally, western blots were conducted to detect the expression level of apoptosis-related Bax and the Bcl-2 proteins. The results suggested that GRg6 can inhibit the proliferation of human lymphocytoma JK cells. GRg6 blocks an S arrest in the cell cycle. With the increase in GRg6 concentration, the potential in the cell decreased in a dose dependent manner, and Bax protein expression gradually increased, whereas Bcl-2 protein expression gradually decreased. In conclusion, GRg6 can inhibit JK cell proliferation in human lymphocytoma and induce its apoptosis. The mechanism of action may be related to mitochondrial dysfunction and an increase of Bax expression and decrease of Bcl-2 expression caused by GRg6.
Highlights
The term lymphocytoma refers to the canceration of lymphocytes, viz. lymphoma
‘World Health Organization neoplasia pathological classification standard of the lymphatic system,’ the nearly 70 kinds of known types of pathological lymphoma can generally be divided into two categories: Hodgkin lymphoma and non-Hodgkin lymphoma
The results indicated that steamed notoginseng extracts could significantly inhibit the activity of SW-480 cell proliferation in human colorectal cancer
Summary
The term lymphocytoma refers to the canceration of lymphocytes, viz. lymphoma. According to the ‘World Health Organization neoplasia pathological classification standard of the lymphatic system,’ the nearly 70 kinds of known types of pathological lymphoma can generally be divided into two categories: Hodgkin lymphoma and non-Hodgkin lymphoma. Shi Sun [1] and other researchers found that the saponin composition of notoginseng changes after steaming. They studied and measured the composition and antitumor activity of notoginsenoside processed with different steaming times and temperatures. The results indicated that steamed notoginseng extracts could significantly inhibit the activity of SW-480 cell proliferation in human colorectal cancer. Toh [2] and other researchers found that ingredients such as ginsenoside Rk3, Rh2, Rg3, and Rk1 abundant in steamed notoginseng can inhibit SNU449, SNU182, and HepG2 hepatocellular carcinoma cell proliferation. A previous report [3] discussed the function of GRg6 in inhibiting platelet aggregation, but no report is available regarding the effect of GRg6 on human lymphocytoma JK cells.
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