Apoptosis In Recovering Human Skeletal Muscle after High Intensity Cycling In Men Receiving Ergogenic Rg1 Compound

Abstract

PURPOSE: To examine the impact of high intensity exercise on cell death in human skeletal muscle during recovery with Rg1 supplementation, which has been shown to increase endurance performance. METHODS: Using randomized double blind placebo controlled crossover design, twelve young men were studied on three occasions: Placebo, Rg1 (1 mg) and Rg1 (5 mg) supplementation 1 h prior to a high-intensity exercise. Biopsied samples were taken from vastus lateralis before, immediately after and 3 h after a 1-h cycling exercise at 75% VO2max. RESULTS: Apoptotic and necrotic cells in vastus lateralis increased immediately after 1 h cycling (80-140% and 80-120%, P < 0.05), concurrent with macrophage infiltration (both CD68+ and CD163+). Increases in iNOS and myogenic factor Myf5 mRNA levels after exercise were further elevated during Rg1 supplemented trial. Rg1 (5 mg) significantly increased high intensity endurance performance and accelerates the disappearance of apoptotic and necrotic cell number occurred 3 h after exercise during recovery. CONCLUSION: High intensity exercise increased cell death in human skeletal muscle after high intensity aerobic exercise. The ergogenic compound Rg1 accelerates phagocytosis to remove unhealthy muscle cell in exercised skeletal muscle.