Abstract
To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancer therapy. We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method). PCDNA3/Apoptin successfully induced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p<0.05). Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy.
Highlights
Apoptin is a kind of viral protein from Chicken Anemia Virus, which can induce apoptosis in various tumor cells, but not in normal cells
Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy
The plasmid PCDNA3/Apoptin was identified with double digestion of BamH I and Xho I, and sequence
Summary
Apoptin is a kind of viral protein from Chicken Anemia Virus, which can induce apoptosis in various tumor cells, but not in normal cells. Methods: We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method).
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