Apophysomycesthailandensis (Mucorales, Mucoromycota), a new species isolated from soil in northern Thailand and its solubilization of non-soluble minerals.

Surapong Khuna,Jaturong Kumla,Saisamorn Lumyong,Nakarin Suwannarach,Wipornpan Nuangmek,Tanongkiat Kiatsiriroat,Jomkhwan Meerak

doi:10.3897/mycokeys.45.30813

Abstract

A new species of soil fungi, described herein as Apophysomycesthailandensis, was isolated from soil in Chiang Mai Province, Thailand. Morphologically, this species was distinguished from previously described Apophysomyces species by its narrower trapezoidal sporangiospores. A physiological determination showed that A.thailandensis differs from other Apophysomyces species by its assimilation of D-turanose, D-tagatose, D-fucose, L-fucose, and nitrite. A phylogenetic analysis, performed using combined internal transcribed spacers (ITS), the large subunit (LSU) of ribosomal DNA (rDNA) regions, and a part of the histone 3 (H3) gene, lends support to our the finding that A.thailandensis is distinct from other Apophysomyces species. The genetic distance analysis of the ITS sequence supports A.thailandensis as a new fungal species. A full description, illustrations, phylogenetic tree, and taxonomic key to the new species are provided. Its metal minerals solubilization ability is reported.

Highlights

The genus Apophysomyces, proposed by Misra et al (1979) with A. elegans as type species, belongs to the family Saksenaeaceae of the order Mucorales (Hoffmann et al 2013)
The present study identifies a new species of Apophysomyces, a soil fungus from Thailand based on morphological and physiological characteristics as well as on phylogenetic analyses
Apophysomyces thailandensis is characterized by its funnel- to bellshaped apophyses and slightly trapezoidal sporangiospores

Summary

Introduction

The genus Apophysomyces, proposed by Misra et al (1979) with A. elegans as type species, belongs to the family Saksenaeaceae of the order Mucorales (Hoffmann et al 2013). This genus is mainly characterized by pyriform sporangia, conspicuous funneland/or bell-shaped apophyses, and subhyaline, smooth-walled sporangiospores (Misra et al 1979; Cooter et al 1990; Alvarez et al 2010). Sutton & Guarro (Alvarez et al 2010), A. trapeziformis E. Cano, Stchigel & Guarro (Bonifaz et al 2014)

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Conclusion