Apolipoprotein A5 and apolipoprotein C3 single nucleotide polymorphisms are correlated with an increased risk of coronary heart disease: a case-control and meta-analysis study.

Guang Yang,Chun-Lei Yu,Xiao-Xiao Liu,Zhe An,Chun-Li Song,Ming-Ming Lei

doi:10.1186/s12944-015-0110-6

Abstract

BackgroundTriglycerides (TGs) are proatherogenic lipoproteins involving the risk of coronary heart disease (CHD), while apolipoprotein A5 (APOA5) and apolipoprotein C3 (APOC3) are main lipoproteins composing TG-rich lipoproteins. In this study, we aim to explore the correlation of CHD with APOA5 -1131 T > C and APOC3 -455 T > C single nucleotide polymorphisms (SNPs).MethodsA sum of 210 CHD patients, hospitalized between Jan. 2013 and Mar. 2015 at China-Japan Union Hospital, Jilin University, were selected as our case group and 223 healthy individuals who had physical examination at same hospital at the same period were selected as control group. The frequency distribution of genotypes of APOA5 -1131 T > C and APOC3 -455 T > C SNPs were measured by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The Stata 12.0 software was utilized for statistical analyses.ResultsThere was no significant difference on age and sex between case and control group (P > 0.05). History of smoking, drinking, hypertension and diabetes mellitus, body mass index and levels of TG and fasting blood sugar in case group were shown to be higher than control group (P < 0.05), while levels of total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol in case group were lower than control group (P < 0.05). Both CC and TC′ + CC frequencies of APOA5 -1131 T > C and APOC3 -455 T > C in case group were higher compared to control group (both P < 0.05). Additionally, T allele frequencies of the two SNPs in case group were lower than control group, while C allele in case group has higher frequencies compared to control group (both P < 0.05). The results of meta-analysis under allele and dominant models showed that APOA5 -1131 T > C and APOC3 -455 T > C SNPs are likely to increase the risk of CHD (both P < 0.05).ConclusionAPOA5 -1131 T > C and APOC3 -455 T > C SNPs may play potent roles in the development and progression of CHD.

Highlights

Coronary heart disease (CHD), known as coronary atherosclerotic heart disease is a kind of thrombotic arterial disease, caused by hypoxia and myocardial ischemia owing to coronary atherosclerotic mediated vascular obstruction as well as coronary functional change [1, 2]
History of smoking, drinking, hypertension and diabetes mellitus, and levels of TG and fasting blood sugar (FBS), and body mass index (BMI) in case group were shown to be higher than control group (P < 0.05), while levels of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in case group were lower than control group (P < 0.05) (Table 2)
Distribution of apolipoprotein A5 (APOA5) -1131 T > C and apolipoprotein C3 (APOC3) -455 T > C single nucleotide polymorphisms (SNPs) Distribution frequencies of genotypes of both APOA5 -1131 T > C and APOC3 -455 T > C SNPs complied with Hardy-Weinberg equilibrium in case and control groups, which indicated that this two SNPs were shown to be representative in this population

Summary

Introduction

Coronary heart disease (CHD), known as coronary atherosclerotic heart disease is a kind of thrombotic arterial disease, caused by hypoxia and myocardial ischemia owing to coronary atherosclerotic mediated vascular obstruction as well as coronary functional change [1, 2]. CHD is a complex multifactorial disorder and both non-genetic and genetic factors can contribute to the development and progression of this disease [5]. Prime interest was devoted to LDL-C as proatherogenic, TG are considered in the last years to be proatherogenic and some studies provide evidence for causal involvement of TG-mediated pathways in CHD and the concentration of it (both fasting and non-fasting) in prediction of future cardiovascular disease events [9,10,11,12,13,14]. Triglycerides (TGs) are proatherogenic lipoproteins involving the risk of coronary heart disease (CHD), while apolipoprotein A5 (APOA5) and apolipoprotein C3 (APOC3) are main lipoproteins composing TG-rich lipoproteins. We aim to explore the correlation of CHD with APOA5 -1131 T > C and APOC3 -455 T > C single nucleotide polymorphisms (SNPs)

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Results

Conclusion