Apolipoprotein A1 rs5070 A/G polymorphism with stroke subtypes in Taiwan

Abstract

BackgroundApolipoprotein A1 (ApoA1) is a structural protein of high-density lipoprotein cholesterol; ApoA1 is involved in lipid and cholesterol metabolism. This study evaluated the association of ApoA1 polymorphism (rs5070) with different stroke subtypes in Taiwanese individuals. MethodsA total of 2139 cases, including 614 controls and 708 large artery atherosclerosis (LAA), 377 small-vessel occlusion, and 440 hypertensive intracranial hemorrhage cases, were enrolled in this study. ApoA1 polymorphism was genotyped through polymerase chain reaction amplification and then subjected to mass-assisted laser desorption ionization time-of-flight mass spectrometry using a Bruker SNP genotyping system in the National Center for Genome Medicine (Academia Sinica, Taipei, Taiwan; http://ncgm.sinica.edu.tw/). ResultsThe frequency of ApoA1 rs5070 dominant genotype (AA vs. AG+GG) was not significantly different among LAA, small-vessel occlusion, and hypertensive intracranial hemorrhage groups compared with that of the control group. Compared with diabetic patients with the AA allele, those with the AG+GG allele of ApoA1 rs5070 polymorphism showed a 1.58-fold likelihood of developing LAA (odds ratio=1.58; 95% confidence interval=1.00–2.42; p=0.046), but not small-vessel occlusion or hypertensive intracranial hemorrhage. In male diabetic patients, the odds ratio increased to 1.90-fold. ConclusionOur findings suggested that genetic polymorphisms of ApoA1 rs5070 A/G may play a role in the susceptibility to LAA among male diabetic patients.