Apolipoprotein A-I inhibits the increased activities of chitotriosidase and β-glucosaminidase in the liver of mice with BCG-induced tuberculosis inflammation

Abstract

The aim of the investigation was to study the activity of lysosomal chitinases ( chitotriosidase and β- glucosaminidase ) in the liver of mice using a model of BCG- induced tuberculous inflammation after intravenous administration of apolipoprotein A-I. Material and methods . The study was carried out on male CBA mice weighing 20–22 g. Disseminated tuberculous inflammation was modeled by a single intraperitoneal injection of 0.5 mg of BCG vaccine . The activity of chitinases was determined using fluorescent substrates 4-methylumbelliferyl β-D- N,N ′,N ′′- triacetylchitotrioside and 4-methylumbelliferyl N- acetyl -β-D- glucosaminide . Results and discussion . BCG- infection of animals after 4 weeks caused a significant increase in the activity of endogenous chitinases in comparison with the control group : chitotriosidase – 3.05 times (p <0.001), β- glucosaminidase – 1.76 times (p <0.01). Intravenous administration of apolipoprotein A-I to animals against the background of BCG infection inhibited the increased enzyme activity , values did not significantly differ from the control values . Conclusions . The results of these studies indicate the ability of apolipoprotein A-I to reduce the increased activity of endogenous lysosomal chitinases in the liver of mice with BCGinduced tuberculous inflammation .