Abstract
Magnetic particle mediated transport in combination with nanomaterial based drug carrier has a great potential for targeted cancer therapy. In this study, doxorubicin encapsulation into the apoferritin and its conjugation with magnetic particles was investigated by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The quantification of encapsulated doxorubicin was performed by fluorescence spectroscopy and compared to CE-LIF. Moreover, the significant enhancement of the doxorubicin signal was observed by addition of methanol into the sample solution.
Highlights
Magnetic particles can be the size of several nanometers to several micrometers and consist mainly of iron, nickel, cobalt and gadolinium [1,2,3,4,5,6]
It has been established that employment of separation technique such as capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is beneficial for apoferritin encapsulated doxorubicin (APODOX) investigation due to the ability to distinguish between encapsulated and desorbed DOX
The targeted therapy is a direction of current anticancer treatment and numerous transporters are searched
Summary
Magnetic particles can be the size of several nanometers to several micrometers and consist mainly of iron, nickel, cobalt and gadolinium [1,2,3,4,5,6]. The material employed for surface coating of the magnetic particles must be nontoxic and biocompatible and has to enable us a targeted delivery with localization in a required area [2,11]. Delivery by magnetic particles can be coupled to specialized nanocarriers such as lipid- [21] and/or protein-based carriers [22,23] enabling selective release of the drug in the site of the action. Such release may be performed by various mechanisms including photo- [24] or thermoiniciated [25] or pH triggered release [23,26]. Magnetic particle-based targeted, apoferritin mediated and pH triggered transport of doxorubicin (DOX) was studied using capillary electrophoresis with laser-induced fluorescence detection
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