Abstract
Four patients on maintenance hemodialysis who have appreciable amounts of apolipoprotein (apo) B-48 in very low density lipoprotein (VLDL) and intermediate density lipoprotein (IDL) were chosed to evaluate the effect of hemodialysis itself on apo B-48 metabolism.After 12-14hrs overnight starvation, venous blood of 15ml was drawn at predialysis and at 2hrs later during hemodialysis and separated into serum which contained 1mM EDTA and 0.01% Tobramycin.The serum was used for chemical analyses and sequential ultracentrifugation to isolate VLDL and IDL. The detection and densitometric scanning of apolipoprotein in those lipoproteins were performed by the staining with Coomassie brilliant blue after 3.5% polyacrylamide-gel electrophoresis in 0.1% sodium dodecyl sulfate.After hemodialysis, serum triglyceride (TG) levels dramatically decreased while serum cholesterol and phospholipid levels remained unchanged. The reduction of serum TG levels resulted in a decrease in VLDL with more protein, cholesterol and less TG on chemical composition, but not in IDL. The apo B-48 of VLDL in all cases and IDL in some cases disappeared after 2hrs hemodialysis.These results suggest that hemodialysis itself has an effect on a removal of apo B-48 in TG-rich lipoproteins in patients on maintenance hemodialysis.
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