Abstract
The aims of this study were to identify the bacterial genera and species present in semen of Apis mellifera and to evaluate the sensitivity of those bacteria to different antibiotics. Mesophilic aerobic and microaerophilic bacteria were characterized in semen samples from different hives, and the number of total viable bacteria per milliliter of semen was determined. Twenty-nine isolates were obtained, and 14 different genera were identified, some of them being monomicrobians and others polymicrobians. Colony-forming units (CFU) were variable from no bacterial development up to 4 × 105 CFU/mL. The most frequent genera were Bacillus and Staphylococcus. All the studied isolates were sensitive to gentamicin and amikacin. Spermatozoa viability in sperm samples diluted in buffer with antibiotic was similar to that of pure semen.
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