Abstract
Background and Aim:Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells.Materials and Methods:Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays.Results:Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells.Conclusion:Exposure to API induced oxidative stress in mouse Sertoli TM4 cells.
Highlights
Over the past few decades, bioactive compounds in plants have attracted increasing interest from the public and scientific community due to their vast therapeutic benefits [1,2,3]
MTS cell proliferation assay kit, lactate dehydrogenase (LDH)-cytotoxicity assay kit II, 2’7’-dichlorofluorescin diacetate (DCFDA) cellular reactive oxygen species (ROS) detection assay kit, lipid peroxidation assay kit, and glutathione reductase (GR) assay kit were purchased from Abcam (Cambridge, MA, USA)
The results indicated that API was cytotoxic to TM4 cells in a time- and concentration-dependent manner, as the half-maximal inhibitory concentration (IC50) values were 95.5, 47.7, and 40.9 μM at 24, 48, and 72 h of incubation, respectively (Figure-1)
Summary
Over the past few decades, bioactive compounds in plants have attracted increasing interest from the public and scientific community due to their vast therapeutic benefits [1,2,3]. Emerging evidence has shown that Sertoli cells are sensitive to harmful substances in food [25], hormonal treatments [25,26,27], and environmental toxicants. These cells are located in the seminiferous tubules of the testis and provide nutrients and structural support for developing spermatids [28,29]; disrupting them through xenobiotic-induced toxicity might compromise male reproductive health and function [30]. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells
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