Abstract
Purpose: To investigate the anticancer activity of apigenin on human cervical cancer cells. Methods: The anti-proliferative effects of apigenin on HeLa cervical cancer cells were determined by 3-(4,5-dimethylthiazol-2-yl)- )-2,5-diphenyltetrazolium bromide (MTT) and clonogenic assays, while its effect on apoptosis was assayed by DAPI and annexin V/PI double staining. Expression of proteins was assessed by immunoblotting. Results: Apigenin exerted anticancer effects on HeLa cervical cancer cells with an IC 50 of 15 μM, and also reduced the colony formation of HeLa cells. These antiproliferative effects were due to induction of apoptosis as indicated by DAPI and annexin V/PI staining. Apigenin altered Bax/Bcl-2 ratio, thereby triggering apoptosis, and also inhibited the Raf/MEK/ERK signalling pathway. Conclusion: These results indicate that apigenin suppresses the growth of cervical cancer cells and may prove to be an important molecule for the treatment of cervical cancer. Keywords: Cervical cancer, Apigenin, Apoptosis, Bax
Highlights
Cervical cancer is one of the most reported cancers among women around the globe [1]
After treatment with the different concentrations of apigenin, apoptosis was determined by DAPI staining
The results showed that apigenin caused apoptosis in a concentration-dependent pattern, as evident from the greater density of white color nuclei (Figure 4)
Summary
Cervical cancer is one of the most reported cancers among women around the globe [1]. The present study was designed to investigate the anticancer activity of apigenin against HeLa cervical cancer cells. Cultured HeLa cervical cancer cells were grown at a density of 1.5×104 in 96-well microtiter plates, and subjected to treatment with apigenin (0-100 μM). This was followed by the addition of MTT solution to all the wells. The plates were kept at 37 ○C for 48 h to permit cell adherence This was followed by the addition of different concentrations of apigenin (0, 7.5, 15 and 300 μM). The HeLa cervical cancer cells were grown in 6well plates (2 × 105 cells per well) for 24 h, and treated with apigenin at 0, 7.5 and 30 μM doses for 24 h. Values of p
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