Abstract

The tumor suppressor gene adenomatous polyposis coli (Apc) is mutated in familial adenomatous polyposis (FAP) and sporadic colorectal tumors. The product of the Apc gene (APC) has been found to bind to β-catenin and human homolog of discs large (hDLG), and to be involved in the Wnt/Wingless signaling pathway. In this study, we examined distribution and subcellular localization of APC and its colocalization with β-catenin and hDLG in the mouse kidney by immunohistochemistry and immunoelectron microscopy. APC was highly expressed throughout the medullary region of the kidney, and localized mainly in the basal cytoplasm of epithelial cells of the thin portion of Henle's loop. It was found that APC was colocalized with β-catenin and hDLG by double-immunolabelling at both light- and electron-microscopic levels. These results suggest that APC complexed with β-catenin and hDLG may be involved in some signaling pathways regulating cellular functions of Henle's loop epithelial cells.

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