Abstract
The pharmacological effects of 4-phenyl-2-trichloromethyl-3H-1, 5-benzodiazepine hydrogen sulfate (PTMB), a novel synthetic benzodiazepine, were examined in mice. In the elevated plus-maze test of anxiety, 0.3-1 mg/kg diazepam ip (F(3,53) = 3.78; P<0.05) and 1-10 mg/kg PTMB ip increased (F(5,98) = 3.26; P<0.01), whereas 2 mg/kg picrotoxin ip decreased (F(3,59) = 8.32; P<0.001) the proportion of time spent in the open arms, consistent with an anxiolytic action of both benzodiazepines, and an anxiogenic role for picrotoxin. In the holeboard, 1.0 mg/kg diazepam ip increased (F(3,54) = 2.78; P<0.05) and 2 mg/kg picrotoxin ip decreased (F(3, 59) = 4.69; P<0.01) locomotor activity. Rotarod assessment revealed that 1 mg/kg diazepam ip and 3, 10 and 30 mg/kg PTMB ip produced significant motor incoordination compared to vehicle control (F(4, 70) = 7.6; P<0.001). These data suggest that the recently synthesized PTMB compound possesses anxiolytic activity and produces motor incoordination similar to those observed with diazepam.
Highlights
The effect of the compounds on spontaneous locomotor activity and exploratory behavior was assessed by the holeboard test, as previously reported [11]
PTMB administration caused a reduction in the number of rearing responses in the holeboard (F(5,98) = 2.57; P
Statistical analysis of plus-maze data revealed that picrotoxin decreased the proportion of time spent in the open arms (F(3,59) = 8.32; P
Summary
The effect of the compounds on spontaneous locomotor activity and exploratory behavior was assessed by the holeboard test, as previously reported [11]. The number of entries and the time spent in closed and open arms was recorded for 5 min. Picrotoxin (2 mg/kg) administration caused a reduction in the locomotor activity of the animals (F(3,59) = 4.69; P
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