Abstract
BackgroundPlatinum derivatives are used widely for the treatment of many cancers. However, the toxicity that is observed makes imperative the need for new drugs, or new combinations. Anvirzel™ is an extract which has been demonstrated with experimental data that displays anticancer activity. The aim of the present study is to determine whether the combination of Cisplatin and Anvirzel™ has a synergistic effect against different types of cancer.Materials and methodsTo measure the efficacy of treatment with Cisplatin and Anvirzel™, methyl-tetrazolium dye (MTT) chemosensitivity assays were used incorporating established human cancer cell lines. Measurements were performed in triplicates, three times, using different incubation times and different concentrations of the two formulations in combination or on their own. t-test was used for statistical analysis.ResultsIn the majority of the cell lines tested, lower concentrations of Anvirzel™ induced a synergistic effect when combined with low concentrations of Cisplatin after an incubation period of 48 to 72 h. The combination of Anvirzel™/Cisplatin showed anti-proliferative effects against a wide range of tumours.ConclusionThe results showed that the combination of Anvirzel™ and Cisplatin is more effective than monotherapy, even when administered at low concentrations; thus, undesirable toxic effects can be avoided.
Highlights
Platinum derivatives are used widely for the treatment of many cancers
AnvirzelTM is an extract of Nerium oleander comprised primarily of Oleandrin and Oleandrigenin [8]
The formulation that elicited the most effective results in most of the cell lines was a combination of 0.01 ng/ ml AnvirzelTM and 0.1 μg/ml Cisplatin, with incubation period of 48 h or 72 h
Summary
Platinum derivatives are used widely for the treatment of many cancers. the toxicity that is observed makes imperative the need for new drugs, or new combinations. The aim of the present study is to determine whether the combination of Cisplatin and AnvirzelTM has a synergistic effect against different types of cancer. Results: In the majority of the cell lines tested, lower concentrations of AnvirzelTM induced a synergistic effect when combined with low concentrations of Cisplatin after an incubation period of 48 to 72 h. The combination of AnvirzelTM/Cisplatin showed anti-proliferative effects against a wide range of tumours. Many studies demonstrate the anti-proliferative activity of Oleandrin. These properties make it attractive for use as a treatment for cancer [1,2,3,4,5]; a major problem is that Oleandrin is toxic to normal cells and tissues [6,7].
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