Anuran mating calling circuits: Inhibition by prostaglandin

Abstract

Male American toads ( Bufo americanus) were induced to mating call in response to electronically simulated, conspecific mating calls. The injection of prostaglandin (PG) F 2α caused suppression of mating call answering. Neural correlates of mating calling were triggered by electrical stimulation of the anterior preoptic nucleus in the isolated brainstem of male, Northern leopard frogs ( Rana p. pipiens). The addition of PGF 2α to the bath completely abolished the correlates of mating calling without changing the correlates of pulmonary respiration. The suppression of mating calling shown here, along with the suppression of release signalling described by Diakow and Nemiroff (1981), supports the hypothesis of a close interrelation between the neural circuits of these two calls. The suppression of the neural correlates of mating calling in an isolated preparation shows a central site of action of the PG. The retention of normal correlates of pulmonary respiration, even after suppression of mating calling correlates, suggests that the generation of mating calling patterns involves the extension and pulsing of the expiratory phase of breathing.