Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P.

Hye Yeon Lim,Jongsung Lee,Yeong-Gyeong Yu,Kon Kuk Shin,Yo Han Hong,Eunji Kim,Sang Hee Park,Jae Youl Cho,Deok Jeong,Hun Kim,Tae-Rahk Kim

doi:10.3390/ijms21051620

Hye Yeon Lim, Jongsung Lee + Show 9 more

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https://doi.org/10.3390/ijms21051620

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Abstract

UVB irradiation can induce generation of reactive oxygen species (ROS) that cause skin aging or pigmentation. Lactobacillus acidophilus is a well-known probiotic strain that regulates skin health through antimicrobial peptides and organic products produced by metabolism and through immune responses. In this study, we investigated the antioxidative, antiwrinkle, and antimelanogenesis effects of tyndallized Lactobacillus acidophilus KCCM12625P (AL). To analyze the effects of AL on UV irradiation-induced skin wrinkle formation in vitro, human keratinocytes and human dermal fibroblasts were exposed to UVB. Subsequent treatment with AL induced antiwrinkle effects by regulating wrinkle-related genes such as matrix metalloproteinases (MMPs), SIRT-1, and type 1 procollagen (COL1AL). In addition, Western blotting assays confirmed that regulation of MMPs by AL in keratinocytes was due to regulation of the AP-1 signaling pathway. Furthermore, we confirmed the ability of AL to regulate melanogenesis in B16F10 murine melanoma cells treated with α-melanocyte-stimulating hormone (α-MSH). In particular, AL reduced the mRNA expression of melanogenesis-related genes such as tyrosinase, TYRP-1, and TYRP-2. Finally, we used Western blotting assays to confirm that the antimelanogenesis role of AL was due to its regulation of the cyclic adenosine monophosphate (cAMP) signaling pathway. Collectively, these results indicate that AL has an antiwrinkle activity in damaged skin and can inhibit melanogenesis. Thus, AL should be considered an important substance for potential use in anti-aging drugs or cosmetics.

Highlights

Skin is consistently in contact with the external environment, protecting the body from harmful factors
The cell viability of HaCaT cells was decreased by UVB irradiation (30 mJ/cm2) and recovered by acidophilus KCCM12625P (AL), implying a cytoprotective effect against cell death caused by oxidative stress (Figure 1c)
The antioxidant effects of AL were found through ABTS radical-scavenging assays [46,47,48], which again demonstrates that AL exerts antioxidant effects (Figure 1d)

Summary

Introduction

Skin is consistently in contact with the external environment, protecting the body from harmful factors. The increase in ROS generated by UV irradiation induces cell death, and increases expression levels of matrix metalloproteinases (MMPs) [4,5,6]. This process is characterized by formation of coarse wrinkles, thickening of skin, and dryness [7,8,9,10]. ROS regulate melanogenesis [11,12], and UVB irradiation stimulates keratinocytes to induce α-melanocyte-stimulating hormone (α-MSH) secretion, which triggers cell signaling in response to melanocortin 1 receptor (MC1R) of melanocytes [6]. Melanogenesis is a major factor affecting quality of life due to aesthetic dissatisfaction

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