Antiviral Effects of a Synthetic Aluminium-Magnesium Silicate on Avian Influenza Virus

Madike Ezeibe,James Eze,Anthony Egbuji,Mary Sanda,Omadi Ijabo,Joeseph Ugonabo,Ijeoma Mbuko,Obianuju Okoroafor,Augustine Ngene,Ikechukwu Eze

doi:10.1038/npre.2011.6509.1

Madike Ezeibe, James Eze + Show 8 more

Open Access

https://doi.org/10.1038/npre.2011.6509.1

Copy DOI

Abstract

AbstractEffects of a synthetic Aluminium-Magnesium Silicate (AMS) on Avian Influenza Virus (AIV) were tested. Equal amounts of H5N1 AIV samples and of AMS were mixed, left one hour, at room temperature before centrifuging. The supernatants were remeasured and tested for viral titre, for Mean Death Time (MDT) and Embryo Mortality Rate (EMR) of chicken eggs. Volumes of the viral samples reduced at rate of 23.4 &177; 5.48 %. Viral titres reduced significantly (P from HA, 73 &177; 32.72 to 1.4 &177;0.43). Also, mortality of infected embryos reduced from 100 % to 65% while MDT of those that died, increased significantly (P = 0.001) from 76 &177; 4.38 to 130 &177;17.27 hours. When incubation with AMS was repeated on portions of the same sample, MDT increased from 64 to 104 hours with the portion incubated once. Two AIV portions on which incubation with AMS was repeated could not kill chick embryos.

Highlights

Avian Influenza Viruses are enveloped, single stranded RNA viruses.Two different protein projections cover their envelopes
Summary of Methods: Samples of the H5N1 Avian Influenza Virus (AIV), isolated from outbreaks of Bird Flu in Nigeria were incubated with the AMS
0.1ml of each supernatant was innoculated into yolk sac of two embryonated chicken eggs to determine Mean Death Time (MDT) and Embryo Mortality Rates (EMR) of infected chicken embryos

Summary

Introduction

Avian Influenza Viruses are enveloped, single stranded RNA viruses.Two different protein projections cover their envelopes. These are the Haemagglutinin (H) and the Nueraminidase (N) antigens. Influenza A viruses are subdivided serologically, into sixteen H and nine N subtypes. All the subtypes have been identified in avian species and they coexist with their natural hosts (water fowls and sea birds) in perfect harmoney 2. Disease outbreaks occur in domestic birds when they come in contact with these carriers or with infected pigs 2. Infected birds shed Influenza viruses in their secretions and excretions.So, domestic birds can get infected when they contact secretions or excretions of infected wild birds or formites 3

Results

Discussion

Conclusion