Abstract
BackgroundRadix Wikstroemiae is a common Chinese herbal medicine. The ethyl acetate fraction of the ethanolic extract of W. indica possesses potent in vitro antiviral activity against respiratory syncytial virus (RSV). This study aims to identify the antiviral components of the active fraction.MethodsThe active fraction of the Radix Wikstroemiae extract was isolated with chromatographic methods such as silica gel, Sephadex LH-20 and semi-preparative high performance liquid chromatography (HPLC) columns. The structures of the isolated compounds were determined based on spectroscopic analyses. The in vitro antiviral activity of the compounds against RSV was tested with the cytopathic effect (CPE) reduction assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.ResultsFour biflavonoids, namely neochamaejasmin B, genkwanol B, genkwanol C and stelleranol, were isolated and characterized. Genkwanol B, genkwanol C and stelleranol, which are stereo isomers of spirobiflavonoids, showed potent anti-RSV activity whereas neochamaejasmin B did not.ConclusionNeochamaejasmin B, genkwanol B, genkwanol C and stelleranol were isolated from Radix Wikstroemiae and the complete absolute configurations of five chiral carbons in stelleranol were substantiated for the first time. Furthermore, the anti-RSV activity of genkwanol B, genkwanol C and stelleranol was reported for the first time.
Highlights
Radix Wikstroemiae is a common Chinese herbal medicine
W. indica tablet, an over-thecounter product made from the aqueous extract of Radix Wikstroemiae, is commercially available in China for the treatment of bronchitis, pneumonia, tonsillitis, parotitis and mastitis according to the Pharmacopoeia of China [2]
The molecular formula of compound IV was determined to be C30H22O11 by HR-electrospray ionization-mass spectrometry (ESI-MS) (m/z 557.10931 [M-H], calcd. for C30H21O11 m/z 557.10893)
Summary
Radix Wikstroemiae is a common Chinese herbal medicine. The ethyl acetate fraction of the ethanolic extract of W. indica possesses potent in vitro antiviral activity against respiratory syncytial virus (RSV). This study aims to identify the antiviral components of the active fraction. (Thymelaeaceae) (Radix Wikstroemiae, Liaogewanggen) is a Chinese herbal medicine used to treat various inflammatory conditions [1]. W. indica tablet, an over-thecounter product made from the aqueous extract of Radix Wikstroemiae, is commercially available in China for the treatment of bronchitis, pneumonia, tonsillitis, parotitis and mastitis according to the Pharmacopoeia of China [2]. W. indica have antifungal, anti-inflammatory, anti-cancer, antiviral and antimalarial effects [3,4,5,6]. The ethyl acetate fraction of the ethanolic extract of Radix Wikstroemiae possessed in vitro antiviral
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