Antiviral, antioxidant, cytotoxic and hypolipidemic activities of fucoidans isolated from Sargassum species

Abstract

Sulphated polysaccharides isolated from the brown algae Sargassum asperifolium, Sargassum dentifolium and Sargassum linifolium have been extracted by cold and hot extract. Using in vitro models antiviral, antioxidant, cytotoxic and hyperlipidemic activities of isolated polysaccharides were investigated. The polysaccharides were composed by sugars (32 – 53% w/w), sulfur (1.7 – 4.7%), ash (5 – 25%) and the hot extracts contained protein (6 – 10.0%). Fucose and galactose represented the main carbohydrates of isolated polysaccharides. The antiviral activity of isolated polysaccharides were evaluated against HCV (genotypes 1 and 4) using host cells Huh 7.5 and HepG2, respectively and cell viability were assayed by trypan blue dye exclusion method. Also, they were assessed against adenovirus type 40 using host cells Hep-2. Polysaccharide isolated from cold Sargassum linifolium showed reduction 60, 16.7 and 60% at non toxic doses 455 µg/ml, 6 µg/ml and 2 mg/ml, respectively. Whereas, hot polysaccharide extract of S. linifolium showed reduction 30, 10 and 60% at non toxic dose 0.5 µg/ml, 3.12 µg/ml and 2 mg/ml, respectively.