Antiviral and Virucidal Activities of N-Cocoyl-L-Arginine Ethyl Ester

Hisashi Yamasaki,Yukiko Suzuki,Kazuko Tsujimoto,A Hajime Koyama,Tsutomu Arakawa,Keiko Ikeda

doi:10.1155/2011/572868

Abstract

Various amino acid-derived compounds, for example, Nα-Cocoyl-L-arginine ethyl ester (CAE), alkyloxyhydroxylpropylarginine, arginine cocoate, and cocoyl glycine potassium salt (Amilite), were examined for their virucidal activities against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2), influenza A virus (IAV), and poliovirus type 1 (PV-1) in comparison to benzalkonium chloride (BKC) and sodium dodecylsulfate (SDS) as a cationic and anionic control detergent and also to other commercially available disinfectants. While these amino acid-derived compounds were all effective against HSV-1 and HSV-2, CAE and Amilite were the most effective. These two compounds were, however, not as effective against IAV, another enveloped virus, as against HSV. Cytotoxicity of CAE was weak; at 0.012%, only 5% of the cells were killed under the conditions, in which 100% cells were killed by either SDS or BKC. In addition to these direct virucidal effects, CAE inhibited the virus growth in the HSV-1- or PV-1-infected cells even at 0.01%. These results suggest a potential application of CAE as a therapeutic or preventive medicine against HSV superficial infection at body surface.

Highlights

There has been an increasing necessity for a multitude of preparedness against infectious diseases
The viruses were propagated in Vero cells in MEM supplemented with 0.5% fetal bovine serum (FBS) or in MDCK cells in MEM supplemented with 0.1% bovine serum albumin (BSA) and acetylated trypsin (4 μg/mL)
We examined the virucidal activity of arginine and amino acid derived compounds (i.e., 0.5 M arginine, 0.5 M ButArg, 0.2% Cocoyl-L-arginine ethyl ester (CAE), 0.2% Amisafe, 0.2% Aminosoap, and 0.2% Amilite) on viruses of three completely different kinds; that is, HSV-1 and HSV-2 (Family of Herpesviridae), influenza A virus (IAV) (Family of Orthomyxoviridae), and poliovirus type 1 (PV-1) (Family of Picornaviridae)

Summary

Introduction

There has been an increasing necessity for a multitude of preparedness against infectious diseases. Various amino acid-derived compounds, for example, Nα-Cocoyl-L-arginine ethyl ester (CAE), alkyloxyhydroxylpropylarginine, arginine cocoate, and cocoyl glycine potassium salt (Amilite), were examined for their virucidal activities against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2), influenza A virus (IAV), and poliovirus type 1 (PV-1) in comparison to benzalkonium chloride (BKC) and sodium dodecylsulfate (SDS) as a cationic and anionic control detergent and to other commercially available disinfectants.

Results

Conclusion