ANTIVIRAL AND ANTI-QUORUM SENSING ACTIVTIES OF LYOPHILIZED AQUEOUS EXTRACT OF PROPOLIS FROM SÉTIF

Aziza Kharchi,Fatin Mohd Ikhsan Natrah,Amel Khellil,Elkhamsa Soltani,Hamid Melouli,Nora Haichour,Samia Mezaache-Aichour,Mohamed Mihoub Zerroug,Abdelhalim Khenchouche

doi:10.55251/jmbfs.5222

Aziza Kharchi, Fatin Mohd Ikhsan Natrah + Show 7 more

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https://doi.org/10.55251/jmbfs.5222

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Abstract

Aqueous extracts are preferentially used in traditional medicine in Algeria. Among these extracts, propolis is used for the treatment of wounds, boils, canker sores, burns. The purpose of this study was to evaluate the antiviral/antiquorum sensing (anti-QS) characteristics of Propolis aqueous extract collected from bee hive near Sétif (east of Algeria) against human pathogenic viruses and gram negative bacteria. First the Maximal Allowable Concentration (MAC) of the lyophilized aqueous extract of propolis was determined to avoid the toxicity of the extract. A human lymphoid cell line that contains Epstein Barr Virus (EBV) genome as an episome (P3HR-1) and HEp-2 infected with Coxsackievirus (CVB4), were selected according to their ability to deliver endemic infectious viruses at high titers. Antibacterial activity was screened by evaluating anti-QS capacity of the extract. Pre-treatment revealed that the MAC of the extract reduces the viral titer of Coxsackievirus by half a log from TCID50 = 105.07 to TCID50 = 104.6, and protected HEp-2 against CVB4 infection. While no antiviral effect on the EBV replication was obtained. The anti-QS capability of the extract was showed against Chromobacterium violaceum strain 026, and by protecting Artemia from Vibrio harveyi BB120 infection.The aqueous extract pre-treatment has not antiviral protection, for DNA viruses. It protected HEp-2 cells against CVB4 infection, degraded Acyl Homoserines lactones (AHL), and protected animal Artemia.

Highlights

In big cities like Algiers, there are herbalists essentially at the level of the markets, and their stalls are frequented by a broad public which goes from the adept diligent, convinced of the benefits of the alternative medicines, to the indigent patient in search of an accessible treatment (Hammiche et al, 2013)
Pre-treatment revealed that the Maximal Allowable Concentration (MAC) of the extract reduces the viral titer of Coxsackievirus by half a log from TCID50 = 105.07 to TCID50 = 104.6, and protected HEp-2 against CVB4 infection
By evaluating its antiviral effect and compared to positive control, the MAC of the extract reduces the viral titer of Coxsackievirus by half a log from TCID50= 105.07 to TCID50 = 104.6

Summary

Introduction

In big cities like Algiers, there are herbalists essentially at the level of the markets, and their stalls are frequented by a broad public which goes from the adept diligent, convinced of the benefits of the alternative medicines, to the indigent patient in search of an accessible treatment (Hammiche et al, 2013). During the two last decades, several studies have explored the capabilities of the aqueous extract of propolis These reported several activities as; antitumor either against malignant transformation by retroviruses (Huleihel and Ishano, 2001) or inhibition of malignant cell lines (Nadjafi et al, 2007), antioxidant (Gülçin et al, 2010), immunomodulatory (Orsatti et al, 2010; Soltani et al, 2017), protective against Ultraviolet A (Butnariu and Giuchici, 2011), antiviral (Bufalo et al, 2009), anticariogenic (Oršolić et al, 2003) and antimicrobial (Domacoski et al, 2010; Monte et al, 2014; Soltani et al, 2017, 2021). In the present study two goals were targeted: (i) The first goal evaluated the cytotoxic/protective power of the lyophilized aqueous extract of Propolis in the presence and absence of virus or bacteria; and (ii) the second goal focused on the investigation of an eventual mode of activity of the extract: its anti quorum sensing (anti-QS) power

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