Abstract
BackgroundSwine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation.MethodsIn this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important swine-origin pathogenic viruses by TCID50 assay. Plaque reduction assay and cell apoptosis assay were also used to test the activity of the peptides. Protection effect of piscidin against pseudorabies virus (PRV) was also examined in mouse model.ResultsPiscidin (piscidin 1), caerin (caerin 1.1) and maculatin (maculatin 1.1) could inhibit PRV by direct interaction with the virus particles in a dose-dependent manner and they could also protect the cells from PRV-induced apoptosis. Among the peptides tested, piscidin showed the strongest activity against PRV. Moreover, in vivo assay showed that piscidin can reduce the mortality of mice infected with PRV.ConclusionIn vitro and in vivo experiments indicate that piscidin has antiviral activity against PRV.
Highlights
Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry
The pathogenic viruses isolated from pigs including pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and rotavirus (RV) are commonly observed in China
In one of our recently published papers, we described the antiviral activity of caerin against porcine epidemic diarrhea virus (PEDV) in vitro [20]
Summary
Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation. Swine-origin virus infection is one bottleneck for the development of the porcine industry worldwide. The efforts to alleviate pig viral diseases have focused on the development of vaccines to enhance the adaptive immunity of the hosts [3, 4]. Several viral diseases have broken out in recent years among pig herds, such as the reemergence of swine-origin influenza in 2009 [5], PED in late 2010 [6] and Pseudorabies in 2012 [7]. There is an urgent need to develop novel potential agents to kill these viruses or block their infection
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