Antiviral Activity of Fridericia formosa (Bureau) L. G. Lohmann (Bignoniaceae) Extracts and Constituents.

Geraldo Célio Brandão,Alaíde Braga Oliveira,Erna G Kroon,José D Souza Filho

doi:10.1155/2017/6106959

Geraldo Célio Brandão, Alaíde Braga Oliveira + Show 2 more

Open Access

https://doi.org/10.1155/2017/6106959

Copy DOI

Abstract

A phytochemical study of Fridericia formosa (Bignoniaceae) ethanol extracts of leaves, stems, and fruits was guided by in vitro assays against vaccinia virus Western Reserve (VACV-WR), human herpes virus 1 (HSV-1), murine encephalomyocarditis virus (EMCV), and dengue virus type 2 (DENV-2) by the MTT method. All the ethanol extracts were active against DENV-2, HSV-1, and VACV-WR with best results for the fruits extract against DENV-2 (SI > 38.2). For VACV-WR and HSV-1, EC50 values > 200 μg mL−1 were determined, while no inhibition of the cytopathic effect was observed with EMCV. Five compounds were isolated and identified as the C-glucosylxanthones mangiferin (1), 2′-O-trans-caffeoylmangiferin (2), 2′-O-trans-coumaroylmangiferin (3), 2′-O-trans-cinnamoylmangiferin (5), and the flavonoid chrysin (4). The most active compound was 2′-O-trans-coumaroylmangiferin (3) with SI > 121.9 against DENV-2 and 108.7 for HSV-1. These results indicate that mangiferin cinnamoyl esters might be potential antiviral drugs.

Highlights

Viral infections represent a current problem accounting for severe damage to human health and economic losses in livestock [1]
A compound with retention time (RT) of 7.8 min was detected in all the ethanol extracts as the major constituent in extracts of stems and fruits
Bioguided fractionation of F. formosa ethanol extracts led to the isolation of five compounds (Figure 2) which were identified by comparison with literature spectroscopic data (1H and 13C NMR, DEPT-135 experiment, COSY, HMQC, HMBC, IR, and MS)

Summary

Introduction

Viral infections represent a current problem accounting for severe damage to human health and economic losses in livestock [1]. Some viral diseases such as dengue or dengue fever (DF), herpes, smallpox, and encephalomyocarditis have a high impact in public health in the tropical and subtropical regions of the world [1]. Dengue virus belonging to the Flaviviridae family, Flavivirus genus, is responsible for Dengue fever (DF) and is considered the most common arboviral disease of humans. It is estimated that 390 million cases occur every year around the world and it is endemic in more than 100 countries, including the Americas, Southeast Asia, and Western Pacific, regions most seriously affected [1, 2]. The need for a safe and efficient approach either for treatment or prevention of DF has been considered a global priority [1, 3]

Results

Discussion

Conclusion