Abstract
Using half-leaf method O,O′-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3- phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K326 (Nicotiana tabacum K326) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.
Highlights
Plant virus is a type of plant disease, known as “plants cancer”
Even in the cell nucleus, nucleic acid (DNA or RNA) replication occurs and coat protein is synthesized in the host cell
In order to investigate the mechanism of action of anti-tobacco mosaic virus (TMV) activity of compound 2009104, we studied its effect on defense-related enzymes (PAL, POD, Superoxide Dismutase (SOD)), evaluated chlorophyll content and determined the impact of TMV coat protein
Summary
Plant virus is a type of plant disease, known as “plants cancer”. The impact of climate anomalies and the areas of crops affected by plant virus disease are on the rise resulting in tremendous economic losses in the world in general and China’s agriculture in particular. Plant virus is an obligate parasite in plant cells. Even in the cell nucleus, nucleic acid (DNA or RNA) replication occurs and coat protein is synthesized in the host cell. Since plants do not have the same immune system as animals, and the virus is absolutely dependent on their hosts, to control plant virus disease and develop plant virus inhibitors appears to be extremely difficult.
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